Effect of Non-surgical Periodontal Therapy on Viruses- HSV, EBV and HCMV using Multiplex PCR in Chronic Periodontitis Patients.
Keywords:
HSV, EBV, HCMV, MULTIPLEX PCR, NON SURGICAL PERIODONTAL THERAPY
Abstract
Periodontitis, the most common periodontal pathology, is a chronic disease orchestrated amongst the infectious organisms and humoral and cellular immune responses of the host.2 Initially only the bacteria like Treponema Denticola, PorphyromonasGingivalisand Tannerella Forsythia were linked with the disease and hence the term periodontal microbiology was recoined as periodontal bacteriology. The hunt for the etiology however did not end here as many patients still did not fall into this domain of causative factors. This tickled the researchers to put on their thinking caps when they pondered over viruses being the unexplained etiology. Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each targets. This technique is indispensable with two or more probes that can be distinguished from each other and recognized simultaneously.
References
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2. SaygunI,SahinS,OzdemirA,KurtisB,YaparM,KubarA,etal.Detectionofhuman viruses in patients with chronic periodontitis and the relationship between viruses and clinical parameters. J Periodontol2002;73:1437-43.
3. Sharma M, Kotwal B, Mahajan N, Kharyal S, Slathia B, Tomar V. Role of Viruses in Periodontal Diseases Research Study. Int J Sci Stud2017;5(7):59-64
4. BilichodmathS,MangalekarSB,SharmaDC,PrabhakarAK,ReddySB,KalburgiNB, et al. Herpesviruses in chronic and aggressive periodontitis patients in an Indian population. J Oral Sci2009;51:79-86.
5. G Chandni, D Deepa. Role of periodontopathogenic virus in periodontal disease: a review
6. EMohammad,WFirdous,WIram,KPrabhjot,NSheeba.PolymeraseChainReaction (PCR): Back toBasics
7. Soben Peter. Essentials of Preventive and Community Dentistry (Public Health Dentistry), 4thEdition.
8. Kazi MM, Bharadwaj R. Role of herpesviruses in chronic periodontitis and their associationwithclinicalparametersandinincreasingseverityofthedisease.EurJDent 2017;11:299-304.
9. Imbronito AV, Grande SR, deFretas NM, Okuda O, Lotufo RFM, Nunes FD (2008) DetectionofEpstein-Barrvirusandhumancytomegalovirusinbloodandoralsamples: comparison of three sampling methods. J Oral Sci 50,25-31.
10. Parra B, Slots J. Detection of human viruses in periodontal pockets using polymerase chain reaction. Oral Microbiol Immunol1996;11:289-93.
11. Contreras A, Slots J. Active cytomegalovirus infection in human periodontitis. Oral Microbiol Immunol 1998;13:225-230.
12. Ling LJ, Ho CC, Wu CY, Chen YT, Hung SL. Association between human herpesviruses and the severity of periodontitis. J Periodontol2004;75:1479-85.
13. Botero JE, Parra B, Jaramillo A, Contreras A. Subgingival human cytomegalovirus correlates with increased clinical periodontal parameters and bacterial coinfection in periodontitis. J Periodontol2007;78:2303-10.
14. Botero JE, Vidal C, Contreras A, Parra B. Comparison of nested polymerase chain reaction(PCR),real-timePCRandviralcultureforthedetectionofcytomegalovirusin subgingival samples. Oral Microbiol Immunol 2008;23:239-44.
15. Wu YM, Yan J, Ojcius DM, Chen LL, Gu ZY, Pan JP. Correlation between infections with different genotypes of human cytomegalovirus and Epstein-Barr virus in subgingival samples and periodontal status of patients. J ClinMicrobiol 2007;45:366570.
16. Rotola A, Cassai E, Farina R, Caselli E, Gentili V, Lazzarotto T, Trombelli L. Human herpesvirus7,Epstein–Barrvirusandhumancytomegalovirusinperiodontaltissuesof periodontally diseased and healthy subjects. J ClinPeriodontol 2008; 35:831–837.
17. ChalabiM,MoghimS,MogharehabedA,NajafiF,RezaieF.EBVandCMVinchronic periodontitis: A prevalence study. Arch Virol 2008; 153:1917-9.
18. Grenier G, Gagnon G, Grenier D. Detection of herpetic viruses in gingival crevicular fluidofpatientssufferingfromperiodontaldiseases:prevalenceandeffectoftreatment. Oral Microbiol Immunol 2009: 24:506–509.
19. S. Jankovic, Z. Aleksic, B. Dimitrijevic, V. Lekovic, P. Camargo, B. Kenney: Prevalence of human cytomegalovirus and Epstein-Barr virus in subgingival plaque at peri-implantitis,mucositisandhealthysites.Apilotstudy.Int.J.OralMaxillofac.Surg. 2011; 40: 271–276.
Published
2021-07-31
How to Cite
Rachalwar, S., Kalburgi, V., Nimmala, S. S. H., Gupta, A., Chakkarwar, Y., & Trivedi, A. (2021). Effect of Non-surgical Periodontal Therapy on Viruses- HSV, EBV and HCMV using Multiplex PCR in Chronic Periodontitis Patients. UNIVERSITY JOURNAL OF DENTAL SCIENCES, 7(3). https://doi.org/10.21276/ujds.2021.7.3.4
Section
Original Research Paper
