Comparative evaluation of antibacterial efficacy of Two shape, TruNatomy files against enterococcus faecalis-an invitro study
Aim: The present study aimed to assess the antibacterial efficiency of Two shape (TS) and TruNatomy files (TRN) files against Enterococcus faecalis.
Materials and Methodology: Forty human mandibular premolars with single roots were collected and decoronated to standardize the lengths. The specimens were then mounted in acrylic resin and E. faecalis suspension was used to contaminate the specimens and they were incubated for three weeks. All the specimens were divided randomly into four groups. Instrumentation was done using Twoshape files (TS): Group I, TruNatomy files (TRN): Group II & Manual Crown Down technique (CD) using Gatesglidden drills and Hand files: Group III with sterile distilled water as irrrigant.Group IV was served as control group. Microbial samples were collected using paper points without instrumentation of the samples and after instrumentation from intracanal environment, which were diluted and later plated on tryptic soy broth (TSB).
Results: Significant difference was seen amongst the experimental groups (P<0.05), before and after instrumentation. TS and TRN resulted in more bacterial reduction than CD technique (P<0.05) whereas TS and TRN were almost similar in their efficacy (P>0.05).
Conclusion: TS and TRN promoted higher bacterial reduction than CD technique, with TS demonstrating the highest antibacterial efficacy.
2. Vatkar NA, Hegde V, Sathe .Vitality of Enterococcus faecalis inside dentinal tubules after ve root canal disinfection methods. Journal of conservative dentistry 2016; 19(5): 445.
3. Baker NA, Eleazer PD, Averbach RE, Seltzer S. Scanning electron microscopic study of the efficacy of various irrigating solutions. Journal of Endodontics, 1975; 1:127-35.
4.Adina-Bianca Piț, Ioana-Amalia Borcean, Ionela-Andreea Vărgatu, Andreea Mai, Maryam Shyblak, Samir Mokdad,et al. Evaluation of the time and efficiency of trunatomy, vdw.rotate, protaper gold and reciproc blue in shaping root canals - an in vitro study. Romanian Journal of Oral Rehabilitation 2020; 12(3).
5. Peters OA, Laib A, Gohring TN, Barbakow F. changes in root canal geometry after preparation assessed by high- resolution computed tomography. Journal of Endodontics 2001; 27:1-6