Mounting media used in Histopathology and Laboratory : Roles and Functions : A Short Review
Keywords:
Mounting medium, Aqueous mounting medium, Resinous mounting medium
Abstract
Mounting is considered as very important step. Various mounting media are there like aqueous mounting media and resinous mounting media. Various researches are done to check which mounting media is best for mounting the histopathological tissues and suitable for different types of microscopy. The mounting media helps in holding the tissue sample in between the slide and the cover slip. The refractive index of the mounting media is also an important factor. In this review article, we are trying to review the various types of the mounting media with special emphasis on their .The data was acquired from earlier published literature and also from searching the different database like Pubmed and Google Scholar.
References
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2. Culling C F A. Handbook of Histopathological techniques: (including museum technique), 2nd ed. The University of Michigan: Butterworths; 1963. p. 146-51.
3. Lynch MJ, Raphael SS. Medical laboratory technology and clinical pathology, 2nd ed. The University of Michigan: W. B. Saunders Co; 1969. P. 934
4. Ravikumar S, Surekha R, Thavarajah R, Mounting Media: An over¬view. JNTR Univ Health Sci 2014;3(5):1-8.
5. Mountants and Antifades. Wright cell imaging facility. Available from https// www. Biocentre.helsinki.fi
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8. Staudt T, Lang MC, Medda R, Engelhardt J, Hell SW.2,2′‐Thiodietha¬nol: A new water soluble mounting medium for high resolution op¬tical microscopy. Microsc Res Tech.2007; 70(1):1-9.
9. Dioni W. About microscopy and chemistry of nail polish. Micscape Magazine, Microscopy UK front page Article library August 2002 Edition; 2002.
10. Lee CJ, Snajberk K, Zavarin E. Chemical composition of the cortical essential oil from Abies balsamea. Phytochemistry 1974 13:179-83
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12. Mound, L.A. & Pitkin, B.R.Microscopic whole mounts of Tbrips (Thysanoptera). Entomologist's Gazette 1972 23: 121-5.
13. Noyes, J. S. Collecting and preserving chalcid wasps (Hymenoptera Chalcidoidea). Journal of Natural History 1982,16: 315-34.
14. Hood, J .D. Microscopical whole mounts of lnsects. Comell University,1940, 57 pp.
15. Green, O.R. Pitfalls, problems and procedures in micropalaeontologica1 preparation and conservation. Geological Curator 1995, 6(4): 157-66
16.Bender, F.Canada balsam. Its preparation and uses. Forestry Branch, Departmental Publications,1967, 1182, 1–7.
17. Lillie, R.D., Zirkle, C. & Greco, J.P.Final report of the committee on histological mounting media. Stain Technology,1953,28, 57–80.
18. Southgate, H.W. XXII.—A suggested substitute for Canada balsam as a mounting medium. Journal of the Royal Microscopical Society,1923, 43, 311–4
19. Spence, D.S. Notes on mounting I. The Microscope. The British Journal of Microscopy and Photomicroscopy. The Entomological Monthly,1939, 4, 29–32 & 44
20. Brown PA. A review of technique used in the preparation, curation, and conservation of microscope slides at the natural history museum London. The Biology Curator 1997;10:1-33.
21.Welsby, F.W. Experiments with Canada balsam. The Microscope. The British Journal of Microscopy and Photomicroscopy. The Entomological Monthly,1951, 8, 255–8.
22.Brunner, C.A. & Blueford, J.R. Restoration of radiolarian strewn slides made with Canada balsam. Micropaleontology,1986,32, 43–5.
23. Essig, E.O. Mounting aphids and other small insects on microscopic slides. Pan-Pacific Entomology,1948, 24, 9–22.
24. Wagstaffe, R. & Fidler, J.H. The preservation of natural history specimens. Vol. 1. Invertebrates. H.F. & G. Witherby, London,1955, 205 pp
25. Kim O. An overview of mounting media for microscopy. Microbehu¬ntermicrocsopy magazine 2016;49:1-5.
26. Rawlins, D.J. Light Microscopy; an introduction to Biotechniques. Bios Scientific publishers, Oxford.1992, 143 pp
27. Imms, A.D. Some methods of technique applicable to Entomology. Bulletin of Entomological Research 1929, 20:165-71.
28. Kannan UP, Ramani P, Natesan A, Sherlin HJ, Gheena S, Abhilasha R.et al. Comparing the quality of castor oil with DPX as a mounting medium. Int J Oro Biol 2017; 1(1): 21-3.
29. Cover slipping and mounting media.Polysciences INC, Chemistry beyond the ordinary. June 2007. Available fromhttps// www.poly¬sciences.com/ TDS 432
30. Russel, C.R. A simple method of permanently mounting rotifer trophi. Journal of the Quekett Microscopy Club 1961, 28, 377–8
31. Jersabek, C.D. The ‘Frank J. Myers Rotifera collection’ at the Academy of Natural Sciences of Philadelphia. Hydrobiologia,2005, 546, 137–40
32. Morrison, W.Aqueous media for microscope slides. Turtox News,1942, 20, 157–8
33. Gray, P. The microtomist’s formulary and guide. Blakiston Company, New York, Toronto,1954, 794 pp.
34. Spence, D.S. Notes on mounting III. The Microscope. The British Journal of Microscopy and Photomicroscopy. The Entomological Monthly,1940b, 4, 113–22
35.Linder, D.H. An ideal mounting medium for mycologists. Science,1929, 70, 430
36. Evans, G.O., Sheals, J.G. & Macfarlane, D. Chapter III Techniques. In: Evans, G.O. (Ed.), The terrestrial acari of the British Isles. Vol. I. Introduction and Biology. British Museum (Natural History), London,1961, pp. 61–88
37.Esser, R.P. Two permanent mounting methods compared after six years. Proceedings of the Helminthological Society of Washington,1974, 41, 1013.
38. Horie, V. Materials for conservation: Organic consolidants, adhesives and coatings. Paperback edition of 2nd edition 2010. Routledge, London, New York, 489 pp.
39.Ossiannilsson, F. “Celochloral”—a new mounting medium for insects. Entomologisk Tidskrift,1958, 79, 2–5.
40.Clark EW, Morishita F. C-M medium; a mounting medium for small insects, mites, and other whole mounts. Science. 1950 Dec 29;112(2922):789-90
41.Perruche, L.Résines artificielles et nouveaux milieux de montagne. Bulletin de la Societé Française de Microscopie,1933, 2, 7–9.
42. Frison, E. Coumarone resin as a mounting medium. The Microscope. The British Journal of Microscopy and Photomicroscopy. The Entomological Monthly, (1952a), 8, 39–42
43.Loveland, R.P. & Centifanto, Y.M.,Mounting media for microscopy. Microscope,1986, 34, 181–241
44.Göke, G. Ein neues Diatomeen-Einschlußmittel mit hoher Brechzahl: Aroclor. Mikrokosmos,1973, 62, 278–81.
45. Göke, G.Natürliche und künstliche Harze als Einschlussmittel für die Mikroskopie. Mikrokosmos,2000, 89, 373–6.
46. Disney, R.H.L. & Henshaw, D.H. de C. Berlese fluid for slide mounting insects. Antenna,1988, 12, 106–7
47.Wirth, W.W. & Marston, N. A method of mounting small insects on microscope slides in Canada balsam. Annals of the Entomological Society of America,1968, 61, 783–4
2. Culling C F A. Handbook of Histopathological techniques: (including museum technique), 2nd ed. The University of Michigan: Butterworths; 1963. p. 146-51.
3. Lynch MJ, Raphael SS. Medical laboratory technology and clinical pathology, 2nd ed. The University of Michigan: W. B. Saunders Co; 1969. P. 934
4. Ravikumar S, Surekha R, Thavarajah R, Mounting Media: An over¬view. JNTR Univ Health Sci 2014;3(5):1-8.
5. Mountants and Antifades. Wright cell imaging facility. Available from https// www. Biocentre.helsinki.fi
6. Lee G. Luna Manual of Histologic and Special Staining Techniques. 2nd ed. New York: The Blakiston Division McGraw Hill Book Co.; 1960
7. Ibidi cells in focus: Mounting medium types for Immunofloures¬cence microscopy, ibidi Gmbh, version 1.0,11, October 2016. Avail¬able from https://www.ibidi.com
8. Staudt T, Lang MC, Medda R, Engelhardt J, Hell SW.2,2′‐Thiodietha¬nol: A new water soluble mounting medium for high resolution op¬tical microscopy. Microsc Res Tech.2007; 70(1):1-9.
9. Dioni W. About microscopy and chemistry of nail polish. Micscape Magazine, Microscopy UK front page Article library August 2002 Edition; 2002.
10. Lee CJ, Snajberk K, Zavarin E. Chemical composition of the cortical essential oil from Abies balsamea. Phytochemistry 1974 13:179-83
11.Eastop, V.F. The aquisitation and processing of taxonomic data. In: Szelegiewicz, H. (Ed.), Evoution and biosystematics of aphids. Proceedings of the International Aphidological Symposium at Jabłonna. 511 April 1981. Wroclaw, Ossolineum,1985 pp. 245–70
12. Mound, L.A. & Pitkin, B.R.Microscopic whole mounts of Tbrips (Thysanoptera). Entomologist's Gazette 1972 23: 121-5.
13. Noyes, J. S. Collecting and preserving chalcid wasps (Hymenoptera Chalcidoidea). Journal of Natural History 1982,16: 315-34.
14. Hood, J .D. Microscopical whole mounts of lnsects. Comell University,1940, 57 pp.
15. Green, O.R. Pitfalls, problems and procedures in micropalaeontologica1 preparation and conservation. Geological Curator 1995, 6(4): 157-66
16.Bender, F.Canada balsam. Its preparation and uses. Forestry Branch, Departmental Publications,1967, 1182, 1–7.
17. Lillie, R.D., Zirkle, C. & Greco, J.P.Final report of the committee on histological mounting media. Stain Technology,1953,28, 57–80.
18. Southgate, H.W. XXII.—A suggested substitute for Canada balsam as a mounting medium. Journal of the Royal Microscopical Society,1923, 43, 311–4
19. Spence, D.S. Notes on mounting I. The Microscope. The British Journal of Microscopy and Photomicroscopy. The Entomological Monthly,1939, 4, 29–32 & 44
20. Brown PA. A review of technique used in the preparation, curation, and conservation of microscope slides at the natural history museum London. The Biology Curator 1997;10:1-33.
21.Welsby, F.W. Experiments with Canada balsam. The Microscope. The British Journal of Microscopy and Photomicroscopy. The Entomological Monthly,1951, 8, 255–8.
22.Brunner, C.A. & Blueford, J.R. Restoration of radiolarian strewn slides made with Canada balsam. Micropaleontology,1986,32, 43–5.
23. Essig, E.O. Mounting aphids and other small insects on microscopic slides. Pan-Pacific Entomology,1948, 24, 9–22.
24. Wagstaffe, R. & Fidler, J.H. The preservation of natural history specimens. Vol. 1. Invertebrates. H.F. & G. Witherby, London,1955, 205 pp
25. Kim O. An overview of mounting media for microscopy. Microbehu¬ntermicrocsopy magazine 2016;49:1-5.
26. Rawlins, D.J. Light Microscopy; an introduction to Biotechniques. Bios Scientific publishers, Oxford.1992, 143 pp
27. Imms, A.D. Some methods of technique applicable to Entomology. Bulletin of Entomological Research 1929, 20:165-71.
28. Kannan UP, Ramani P, Natesan A, Sherlin HJ, Gheena S, Abhilasha R.et al. Comparing the quality of castor oil with DPX as a mounting medium. Int J Oro Biol 2017; 1(1): 21-3.
29. Cover slipping and mounting media.Polysciences INC, Chemistry beyond the ordinary. June 2007. Available fromhttps// www.poly¬sciences.com/ TDS 432
30. Russel, C.R. A simple method of permanently mounting rotifer trophi. Journal of the Quekett Microscopy Club 1961, 28, 377–8
31. Jersabek, C.D. The ‘Frank J. Myers Rotifera collection’ at the Academy of Natural Sciences of Philadelphia. Hydrobiologia,2005, 546, 137–40
32. Morrison, W.Aqueous media for microscope slides. Turtox News,1942, 20, 157–8
33. Gray, P. The microtomist’s formulary and guide. Blakiston Company, New York, Toronto,1954, 794 pp.
34. Spence, D.S. Notes on mounting III. The Microscope. The British Journal of Microscopy and Photomicroscopy. The Entomological Monthly,1940b, 4, 113–22
35.Linder, D.H. An ideal mounting medium for mycologists. Science,1929, 70, 430
36. Evans, G.O., Sheals, J.G. & Macfarlane, D. Chapter III Techniques. In: Evans, G.O. (Ed.), The terrestrial acari of the British Isles. Vol. I. Introduction and Biology. British Museum (Natural History), London,1961, pp. 61–88
37.Esser, R.P. Two permanent mounting methods compared after six years. Proceedings of the Helminthological Society of Washington,1974, 41, 1013.
38. Horie, V. Materials for conservation: Organic consolidants, adhesives and coatings. Paperback edition of 2nd edition 2010. Routledge, London, New York, 489 pp.
39.Ossiannilsson, F. “Celochloral”—a new mounting medium for insects. Entomologisk Tidskrift,1958, 79, 2–5.
40.Clark EW, Morishita F. C-M medium; a mounting medium for small insects, mites, and other whole mounts. Science. 1950 Dec 29;112(2922):789-90
41.Perruche, L.Résines artificielles et nouveaux milieux de montagne. Bulletin de la Societé Française de Microscopie,1933, 2, 7–9.
42. Frison, E. Coumarone resin as a mounting medium. The Microscope. The British Journal of Microscopy and Photomicroscopy. The Entomological Monthly, (1952a), 8, 39–42
43.Loveland, R.P. & Centifanto, Y.M.,Mounting media for microscopy. Microscope,1986, 34, 181–241
44.Göke, G. Ein neues Diatomeen-Einschlußmittel mit hoher Brechzahl: Aroclor. Mikrokosmos,1973, 62, 278–81.
45. Göke, G.Natürliche und künstliche Harze als Einschlussmittel für die Mikroskopie. Mikrokosmos,2000, 89, 373–6.
46. Disney, R.H.L. & Henshaw, D.H. de C. Berlese fluid for slide mounting insects. Antenna,1988, 12, 106–7
47.Wirth, W.W. & Marston, N. A method of mounting small insects on microscope slides in Canada balsam. Annals of the Entomological Society of America,1968, 61, 783–4
Published
2022-04-27
How to Cite
DR. HIMANSHU SINGH, DR.VEDANT PATEL, Dr.Rahul A Razdan, DR.PRACHI NAYAK, DR.SUSHRUTH NAYAK, & DR.KUNDENDU ARYA BISHEN. (2022). Mounting media used in Histopathology and Laboratory : Roles and Functions : A Short Review. UNIVERSITY JOURNAL OF DENTAL SCIENCES, 8(3). https://doi.org/10.21276//ujds.2022.8.3.21
Section
Review Article
