Evaluation of Cymbopogon Citratus as Disinfectant and its Effect on the Dimensional Stability of the Resultant Gypsum Casts: An in Vitro Study.
BACKGROUND AND AIM: Proper disinfection of impression is probably the most important procedure for dental lab to render it safe for further handling without affecting the accuracy of fine details. In light of these intermingling facts, author aimed to 1) investigate the antimicrobial effect of Cymbopogon citratus on selected microorganism colony and 2) the effect of the disinfectant solution on the dimensional accuracy of the resultant gypsum casts. MATERIALS & METHOD: Initially, tropicalgin Alginate was mixed and total of 20 impressions of the alginate and painted with bacterial colonies. Swabs were further subjected to culture and sensitivity tests for predetermined bacterial strains. Alginate impressions were immersed in glutaraldehyde 2% and Cymbopogon Citratus 30% for 10 minutes (ten each). The swabs were wiped on glass Petri dish and inoculated. The colonial growth was transferred to the glass slide for Gram staining which confirmed presence of Staphylococcus aureus, Streptococcus mutans and Candida albicans in control group. Cross-arch distance between 16 and 26 of the poured samples were compared with the cross arch distance in the typhodont teeth using digimatic vernier caliper. RESULT: Statistical analysis was attempted using SPSS version 22.0. The results of dimensional accuracy of 2% Glutaraldehyde (Group A) mean reading was 55.114, Variance =0.00687 and standard deviation were 0.8289 obtained. The results of dimensional accuracy of 30% Cymbopogan Citratus (Group B) mean reading was 55.403, Variance = 0.0268 and standard deviation were 0.16384 obtained. CONCLUSION: Within the limitations of the study, authors concluded that anti-microbial efficacy of 2% Glutaraldehyde was less in comparison to 30% Cymbopogon Citratus on resultant selected microorganism in this study and dimensional accuracy was superior in Cymbopogan citratus in comparison to glutaraldehyde.
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